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Commercially offered thermostable Taq ligases are Utilized in amplification reactions as a consequence of their thermostable Houses.
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DNA ligases became indispensable tools in contemporary molecular biology research for generating recombinant DNA sequences. For example, DNA ligases are employed with restriction enzymes to insert DNA fragments, often genes, into plasmids.
It is 2 domain ligase, the primary one would be the adenylation or nucleotide-binding domain. This area serves site for ATP binding, which is found inside a pocket beneath among the list of β-sheets.
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In this method, two ATP molecules are eaten for every one phosphodiester bond. The reaction can commence in a few ways or catalytic gatherings.
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